Editorial overview: Biophysical and molecular biological methods: Structure, dynamics, and single molecules.

A sea change underway in determination of atomic-level structures of macromolecules and complexes is addressed in three reviews on X-ray crystallography and electron microscopy. In crystallography, new, highspeed, low-noise detectors have revolutionized data collection practice. This is combined with new statistical methods to evaluate data and determine diffraction limits and new methods for phase determination without recourse to specific chemical labels. Meanwhile, new microscopes and detectors together with improved methods for sample preparation have vastly expanded the resolution limit and reduced the macromolecule size that is accessible by electron cryo-microscopy (cryo-EM).